The subject provides an overview of fundamental concepts in biochemistry, which focuses upon the living organisms and major biomolecules in living systems. Important topics include the structure and function of water, amino acids, proteins, carbohydrates, lipids, nitrogen and amino acids. The application of buffers as well as enzyme kinetics will be extensively emphasized. Various metabolisms for carbohydrates, lipids, nitrogen and amino acids will be discussed in detail. The course will also emphasize on the laboratory skills which includes introduction to biochemistry analysis (DNS calorimetric method and Lowry method), and enzyme kinetic analysis.